Question: You Perform A Serial Dilution As Shown In The Figure Below: Step #1: Transfer 1.0ml From The Original Culture Into Tube #1 Containing 99ml Of Diluent; Step #2: Inoculate Plate A With 0.1ml Of Inoculum From Tube #1; Step #3: Transfer 1.0ml From Tube #1 Into Tube #2 Containing 99ml Of Diluent; Step #4: Inoculate Plate B With 0.1ml Of Inoculum From Tube …

Question: You Perform A Serial Dilution As Shown In The Figure Below: Step #1: Transfer 1.0ml From The Original Culture Into Tube #1 Containing 99ml Of Diluent; Step #2: Inoculate Plate A With 0.1ml Of Inoculum From Tube #1; Step #3: Transfer 1.0ml From Tube #1 Into Tube #2 Containing 99ml Of Diluent; Step #4: Inoculate Plate B With 0.1ml Of Inoculum From Tube …

You perform a serial dilution as shown in the figure below:

Step #1: transfer 1.0ml from the original culture into tube #1containing 99ml of diluent;
Step #2: inoculate plate A with 0.1ml of inoculum from tube#1;
Step #3: transfer 1.0ml from tube #1 into tube #2 containing 99mlof diluent;
Step #4: inoculate plate B with 0.1ml of inoculum from tube#2;
Step #5: transfer 0.1ml from tube #2 into tube #3 containing 9.9mlof diluent;
Step #6: inoculate plate C with 1.0ml of inoculum from tube#3;
Step #7: transfer 0.1ml from tube #3 into tube #4 containing 9.9mlof diluent;
Step #8: inoculate plate D with 1.0ml of inoculum from tube #4.

What are the total dilution factors for tubes #1 & #3, andthe final dilution values for plates A & C? (Note: write youranswer as 10X, so if an answer is 108, youwould enter 8, and if an answer is 10-8, you would enter-8)

1ml 1ml 0.1ml 0.1ml U Original Culture Tube #1 Tube #2 Tube #3 Tube #4 Diluent Volume: 9ml 9ml 9.9ml 9.9ml Volume Plated: 1 0

1. The total dilution factor in tube #1 = 10 ___________
2. The total dilution factor in tube #3 = 10___________
3. The final dilution value for plate A = 10___________
4. The final dilution value for plate C = 10___________

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1ml 1ml 0.1ml 0.1ml U Original Culture Tube #1 Tube #2 Tube #3 Tube #4 Diluent Volume: 9ml 9ml 9.9ml 9.9ml Volume Plated: 1 0.1ml 10.1ml 11 | 1ml 1ml Plate Plate Plate A Plate с Plate B D