Question: I’m Thinking Of Enyzeme CaC81 Because Its The Closest To The Lacz Gene. But I’m Not Completely Sure As To What The Second Enyzeme Might Be. Thank You For Your Help!Hopefully This Will Help? Since Its All I Have, But Thanks For Helping!

4. Finally, you’ve finally grown up several bacterial colonies that you believe contains the recombinant plasmid, pBluescript with the ADE2 PCR product inserted. You isolate the plasmid DNA (via mini prep) and want to check that the plasmid really contains your insert and see which way the insert is orientated; for example: whether the 5′ end of the ADE2 PCR product is closer to the beginning of the lacZ gene or the 13. promoter A.) Which of the following enzymes would you choose that would be easiest to confirm both, that ADE2 is inserted in the plasmid and it’s orientation in the plasmid Cac81, Dralll, Drdi. Xmnl, Tatl, Neol? (Hint: diagram both pBluescript and your ADE2 PCR fragment for each enzyme and the basepair Wat which the enzyme cuts. Also include the nucleotide(s) where you expect the insert to be put into pBluescript- this was dependent on the enzyme or enzymes you “placed” into your primers.) Drdl: 176-18€ be B.) Briefly, why will each of the other enzymes not be as useful? BlueScrit ROL2 COCS1692-95, 50 – 857,1954 -1367 bp XA1: 49-506, 147-1650 hp • Dral: 223-231 hp • Tod: 520 – 525, 039 – 1043 bp *Cacsi: 828 – 334, 39-394,865-31, “cost TO THE UNCO) 505-56 625-6359-625 6542-4*1*,945-450,993-29, 1652-1037, Camp) 1513-1519 bp Tat: 1941-by • Xmni: pr) 1996-2008 bp 8 15 M power Pharma 176 Drdi (1) 222 Drall (1) 328 Cacal (12) 1998 Xmnl (1) 371 Cac81 (12) 385 CacBI (12) 505 Cac8I (12) 1881 Tall (1) 525 Cac8l (12) SP21 BIO201 USE ME pBluescript pks 529 Cac8l (12) bla AmpR 2318 bp 619 Cac BI (12) LACZ Pronation Nung Site 1513 Cac8l (12) T3 Promoter initiation Site 792 Cacél (12) Lac Promotor 944 Caci (12) 973 CacBl (12) 1082 Cacol (12)