Question: Based On The Info Above, Which Plates Do You Compare In Order To Detect The Activity Of The Ligase? Explain. Can You Tell, Based On The Results, Whether The Ligase Enzyme Worked? Which Plates Do You Compare In Order To Detect The Activity Of The CIP? Explain. Can You Tell, Based On The Results, Whether The CIP Enzyme Worked? Which Transformation(s) …

Question: Based On The Info Above, Which Plates Do You Compare In Order To Detect The Activity Of The Ligase? Explain. Can You Tell, Based On The Results, Whether The Ligase Enzyme Worked? Which Plates Do You Compare In Order To Detect The Activity Of The CIP? Explain. Can You Tell, Based On The Results, Whether The CIP Enzyme Worked? Which Transformation(s) …

885 (blue) 21 (blue) T3 Tube # dilution # mis type of plate: Number of Transform- Average total plated Colonies on ants/ml (t

based on the info above,

which plates do you compare in order to detect the activity ofthe ligase? Explain. Can you tell, based on the results, whetherthe ligase enzyme worked?

which plates do you compare in order to detect the activity ofthe CIP? Explain. Can you tell, based on the results, whether theCIP enzyme worked?

which transformation(s) resulted in the production of whitecolonies? Why?

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885 (blue) 21 (blue) T3 Tube # dilution # mis type of plate: Number of Transform- Average total plated Colonies on ants/ml (transform- transform- the plate ants/ml) ants T1 None 0.1 No Antibiotic 1659 (no (White) DNA) T1 None 0.1 Amp + IPTG (No Growth) (no +X-gal XXXXXXXX XXXXXXXX XXXXXXX DNA) 10(-1) 0.1 Amp + IPTG T2 +X-gal (Native) 10(-2) 0.1 Amp + IPTG 103 (blue) +X-gal None 0.1 Amp + IPTG +X-gal (RC) None 0.2 Amp + IPTG 45 (blue) +X-gal None 0.1 Amp + IPTG 209 (blue) T4 +X-gal (LC) None 0.2 Amp + IPTG 425 (blue) +X-gal None 0.1 Amp + IPTG 2 (blue) T5 +X-gal (PC) None 0.2 Amp + IPTG 10 (blue) +X-gal None 0.05 Amp + IPTG 77 (26G+,22G- +X-gal ,29 blue) None 0.1 Amp + IPTG 159 (55G+,45G. T6 +X-gal ,59 blue) (L) None 0.2 Amp + IPTG 250 (73G+,61G. +X-gal ,116 blue) None 0.2 Amp + IPTG 241 (82G+,69- +X-gal ,90 blue) Note: Blue = Blue colonies on the plates G+ = GFP+ (Whites colonies that are glowing GREEN under UV) and G- = GFP-(Whites colonies that are NOT glowing GREEN under UV)