Question: 622 527 13S 5′-exon 12S 5′-exon 404 309 242 238 217 201 190 180 160 147 95 5′-exon 122 -3′-exon 110 90 1 2 3 4 5 6 7 Figure 14.13 Results Of RNase Protection Assay. Zhuang And Weiner Tested The Wild-type And Mutant 5′-splice Sites And Wild-type And Mutant U1 Sn RNAs Pictured In Figure 14.12 By Transfecting Hela Cells With Plasmids Containing These Genes, …

622 527 13S 5′-exon 12S 5′-exon 404 309 242 238 217 201 190 180 160 147 95 5′-exon 122 -3′-exon 110 90 1 2 3 4 5 6 7 Figure 14.13 Results of RNase protection assay. Zhuang and Weiner tested the wild-type and mutant 5′-splice sites and wild-type and mutant U1 sn RNAs pictured in Figure 14.12 by transfecting Hela cells with plasmids containing these genes, then detected splicing by RNase protection as illustrated in Figure 14.11. Lane 1, size markers, with lengths in base pairs indicated at left. Lane 2, mock-transfected cells (negative control). Lane 3, wild-type E1A gene with wild-type U1 SnRNA. Signals were visible for the 135 and 12S products, but not for the 98 product, which normally does not appear until late in infection. Lane 4, mutant hr440 with an altered 12S 5′-splice site. No 128 signal was apparent. Lane 5, mutant hr440 plus mutant U1 snRNA (U1-4u). Splicing at the 128 5′-site was restored. Lane 6, mutant pm1114 with an altered 135 5′-splice site. No 13S signal was apparent. Lane 7, mutant pm1114 plus mutant U1 snRNA (U1-6a). Even though base pairing between the 5′-splice site and U1 SnRNA was restored, no 13S solicing occurred Su Zhu Y and AM Weer. A comprasatory 4 What is preferred s’ splice sites for wild type E14 in order of their usage and efficiency? a) 95 most, then 12s, and last 135. b) 125 most, then 135, and last as. c.) 13s most, then 125, and last gs. d.) 13s most, then as, and last 125.