Question: 1. For Each Of The Following, Explain How Eukaryotic Transcriptional Initiation Would Be Affected. A. TFIIB B. TEID C. TEIIH 2. If The Following RNA Polymerases Were Missing From A Eukaryotic Cell, What Types Of Genes Would Not Be Transcribed? A. RNA Polymerase ! B. RNA Polymerase II C. RNA Polymerase III 3. How Will Transcription In Prokaryotes Be …
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1. For each of the following, explain how eukaryotic transcriptional initiation would be affected. a. TFIIB b. TEID C. TEIIH 2. If the following RNA polymerases were missing from a eukaryotic cell, what types of genes would not be transcribed? a. RNA polymerase ! b. RNA polymerase II C. RNA polymerase III 3. How will transcription in prokaryotes be affected if a. the -10 sequence in the promoter is deleted b. there is a mutation in the sigma subunit C. there is a mutation in the beta subunit 4. The coding strand of DNA has the sequence 5′ ATGATACTAAG3′ Write the sequence for the template strand. Label 5′ and 3 Write the sequence of the RNA. Label 5′ and 3′ 5. Strand1 Direction of unwinding 3′ с Strand 2 b The diagram shows a replication fork. What is the polarity of i. a b c ii. Which strand is the template for the leading strand? Which strand is the template for the lagging strand? On which strand are Okazaki fragments made? iv. 6. Below represents a promoter in a transcription unit —–TATAAT—-strand 1 —–ATATTA—-strand 2 Fill in the polarity of the strands? Which strand is the coding strand? Which strand is the template strand? What is the direction of transcription? 7. Below represents a promoter in a transcription unit ..—TAATAT ….ATTATA….. Fill in the polarity of the strands? Which strand is the coding strand? Which strand is the template strand? What is the direction of transcription? 8. —-ATTATA— —-TAATAT- Fill in the polarity of the strands? Which strand is the coding strand? Which strand is the template strand? What is the direction of transcription? 9. You have a nucleic acid with the following percentages of bases 1. A=35% G=32% T=8% C=25% II. A=20% G=32% T=18% C=30% III. A=23% G=14% U=42% C=21% For the 3 nucleic acids above, determine whether it is single stranded or double stranded DNA or RNA 10. An RNA molecule has the following percentages of bases A=16% G=27% U=37% C=20% What would be the percentage of bases in the template strand of the DNA that contains the gene for this RNA?
